Figure EV4. More characterization of metabolomics.

• A
  Principal component analysis of analyzed metabolites in DMSO‐treated samples and R406‐treated samples on d8. n = 6.
• B
  Heatmap showing the normalized abundance (z score) of the differential metabolites between DMSO‐treated and R406‐treated samples. Red indicates upregulated, whereas blue indicates downregulated metabolites. n = 6.
• C
  Abundance of R406‐upregulated metabolites in glycine, serine, and threonine metabolism in cells treated with DMSO and R406. n = 6.
• D
  Stable isotope tracing mass spectrometry using ¹³C‐labeled Serine in cells treated with DMSO and R406. n = 3.
• E–G
  Sall4⁺ colony number on d12 after treatments with l‐serine, l‐glycine, and l‐threonine. n = 5.

Data information: All data are presented as mean ± SD. Statistical significance was assessed by the two‐tailed Student’s t‐test, *P < 0.05, ***P < 0.001.