Figure EV5. Further characterization of H₂S on chemical reprogramming.

• A
  H₂S levels in DMSO‐ and R406‐treated reprogramming intermediates by methylene blue method. n = 9.
• B
  Sall4⁺ colony number on d12 after treatments of NAC and R406. n = 5.
• C, D
  GFP⁺ colony number of samples treated with NAC (C) and HA (D) on d8 of TF‐induced reprogramming. n = 5.
• E
  The effect of NAC in human reprogramming. n = 4.
• F
  Diagram showing the procedure of shCth virus infection at the early stage of reprogramming.
• G
  RT–qPCR analysis of Cth expression in MEFs infected with shNC and shCth viruses. n = 3.
• H, I
  Immunofluorescence of Sall4 in shNC‐ and shCth‐infected cells with and without R406 treatment on d12. n = 5. Scale bar, 100 μm.

Data information: All data are presented as mean ± SD. Statistical significance was assessed by the two‐tailed Student’s t‐test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.