Skip to main content
. 2021 May 18;12:644225. doi: 10.3389/fphar.2021.644225

FIGURE 1.

FIGURE 1

The effects of APN on LPS-induced H9c2 cytotoxicity; cardiomyocyte apoptosis; and Bcl2, Bax, cleaved caspase-3 and cleaved caspase-9 expression in H9c2 cells. (A) APN can reduce LPS-induced cytotoxicity. The cells were pre-treated with three concentrations of APN (0.5, 1 and 2 μg/ml) for two hours to assess the preservation function of APN on H9c2 cytotoxicity induced by LPS. (B) Cell morphology was observed under an inverted microscope (magnification, ×40). The CCK-8 assay was used to determine cell viability. (C) LPS downregulated Bcl2 and upregulated Bax, cleaved caspase-3 and cleaved caspase-9 in H9c2 cells, but these effects were attenuated by APN. (D–G) Quantification of Bax, Bcl2, cleaved caspase-3 and cleaved caspase-9 expression. (H) The apoptosis rate after treatment with APN and LPS. (I) Statistical analysis of the apoptosis rate (*p < 0.05, **p < 0.01, ***p < 0.001 vs. control; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. the LPS group. The data are shown as the means ± SEs (n = 6)).