Extended Data Fig. 3. Loss of Stat3 diminished alveolar organoid formation.

(A) PCR shows the expected 802bp product when corn oil is administered to Sftpc^CreERT2:Stat3^LoxP/LoxP mice and the recombined 514bp product when tamoxifen is administered to Sftpc^CreERT2:Stat3^LoxP/LoxP mice. The gels in this figure represent n=2 mice per condition (corn oil and tamoxifen). This experiment was repeated 4 times with similar results. (B) AT2 cells from tamoxifen treated Sftpc^{CreERT2-Rosa26tdTomato} and Sftpc^{CreERT2-Rosa26tdTomato}:Stat3^LoxP/LoxP mice were isolated and co-cultured with PDGFRα mesenchymal cells in transwells containing Matrigel for four weeks (n=4 wells per condition). Organoid forming efficiency was significantly decreased in the absence of STAT3 signaling in AT2 cells (p=2.2x10⁻⁸). Data is shown as the mean +/− SEM. Statistical significance was determined using a two-tailed Student’s t-test. Scale bars, 2.5mm.