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. Author manuscript; available in PMC: 2021 Jun 15.
Published in final edited form as: Dev Biol. 2020 Mar 10;462(2):119–128. doi: 10.1016/j.ydbio.2020.02.016

Fig. 2. The optic stalk is defective in Arl13−/− embryos.

Fig. 2.

Immunostaining against laminin (LAMA1) indicates the basal polarity of the optic stalk in Arl13b−/− embryos (B), white arrow indicates the optic stalk. In Arl13b+/− embryos, Pax2 is expressed in the central retina and optic stalk (C), instead, in Arl13b−/− embryos in addition to the optic stalk, Pax2 is also ectopically expressed in the proximal segment of the inner layer of the optic cup (C′, C″). Immunohistochemistry against Tbx5 and Pax2 in E10.5 Arl13b+/− (D-D″) and Arl13b −/− (E-E″) optic cups. In the mutant embryos Pax2 expression is dorsally expanded and that of Tbx5 is missing (white arrowheads indicate the putative dorsal eye territory). Scale bar: 100 μm. (N: 3).