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. 2021 May 15;11(5):2062–2080.

Figure 2.

Figure 2

PFK-15 arouses apoptotic cell death. A. After 24 h exposure to PFK-15, cell lysates were collected and subjected to immunoblotting with indicated antibodies. B. Flow cytometry was performed after 24 h treatment with PFK-15 to analyze apoptosis (Annexin V-positive). C. Cell viabilities were analyzed by MTS assay in SW480 and HT29 cells after 24 h upon to PFK-15 treatment with or without Z-VAD-FMK (20 μM). D. Cell lysates were subjected to immunoblotting after treatment with 6 μM PFK-15 (unless otherwise indicated) with or without Z-VAD-FMK. **P < 0.01 versus control, and NS indicated of none significant.