Figure 4.

Necroptosis inhibitors attenuate the cell viability loss aroused by PFK-15. (A and B) MTS assays were performed in SW480 and HT29 cells exposed to PFK-15 treatment with or without necroptosis inhibitors (3 μM GSK872, 3 μM NSA). (C and D) Wound healing assay and colony growth assay were performed with PFK-15 (C: 6 μM; D: 1.5 μM) with or without Nec-1. (Scale bars: D, 0.5 mm; E, 1 cm). (E) Following indicated treatment for 24 h, cell lysates of SW480 and HT29 cells were subjected to immunoblotting with apporpriated antibodies. **P < 0.01 versus control, and NS indicated of none significant.