Figure 5.

Siliencing of either RIP1 or RIP3 lessens the cytotoxicity of PFK-15. Cells were transfected with the RIP1, RIP3 target siRNAs or control siRNA for 48 h. (A) Immunoblotting with indicated antibodies was performed to verify the knockdown efficiency. (B and C) MTS assay was performed to monitor the cell viability after 24 h treatment (B: SW480; C: HT29). (D and E) Colony growth assay and wound healing assay were performed with PFK-15 (D: 1.5 μM; E: 6 μM). (Scale bars: D, 1 cm; E, 0.5 mm). (F) Following PFK-15 treatment for 24 h, cell lysates were subjected to immunoblotting. **P < 0.01 versus control, and NS indicated of none significant.