Figure 1.

Downregulation of miR-22 in T cells of HCC patients. A. qPCR analysis of miR-22 expression in 30 pairs of liver normal and malignant tissues. U6 was used as a loading control, P=0.0036. B. Differential expression of miR-22 in different stage of patients with HCC. C. The correlation analysis between miR-22 expression and prognosis of HCC patients. The patients were divided based on the median expression of miR-22 in malignant tissues. D. The correlation analysis between miR-22 expression and prognosis of HCC patients from TCGA data base. The patients were divided based on the average expression of miR-22 in malignant tissues. E. qPCR analysis of miR-22 expression in circulating T cells from HCC patients (n=29) and healthy controls (n=11). U6 was used as a loading control, P<0.0001. F. Differential expression of miR-22 in circulating T cells from HCC patients with (n=14) or without metastasis (n=15). (P=0.0033).