Fig. 8.

ORMDL3 deletion increases S1P lyase activity. A: SPL activity was measured in lysates from control (CTL1), ORMDL3 single KO (ORMDL3 KO1), and triple KO ORMDL1,2,3 (TKO) cells. Data are the mean ± SEM of 2–3 technical replicates from five independent experiments. ∗P < 0.05 compared with CTL determined by one-way ANOVA followed by Tukey’s post hoc analysis. B: cell lysates were analyzed by Western blotting with anti-SPL and anti-GAPDH antibody for equal loading and transfer. A representative blot is shown and relative densities of the immunopositive bands normalized to GAPDH quantified. Data are the mean ± SEM of six biological replicates. S1P, sphingosine-1-phosphate; SPL, S1P lyase.