TABLE 1.

Sequences of PCR primers, guide RNAs, and siRNAs used in this study

RT‐qPCR primers	Forward (5′‐3′)	Reverse (5′‐3′)
FOLR1	TTCATCCAGGACACCTGCCTC	ATTGCTCACAGTCCTCTTTGC
MDM2	AACAGGTGTCACCTTGAAGGTG	TGAGGTAGATGGTCTAGAAACC
GAPDH	TGGTAAAGTGGATATTGTTGC	TTCTCAGCCTTGACGGTGC
Genotyping PCR primers
FOLR1	TCAGGTGATCCACCCACCTC	AGATCTTTGGAGGAGTCATTC
Guide RNAs	Sense (5′‐3′)	Antisense (5′‐3′)
FOLR1‐1	ACACC ACCTGAACCTCGTGACCACC G	AAAAC GTGGTCACGAGGTTCAGGTC G
FOLR1‐2	ACACC GTTGGCATTGTACCGACATT G	AAAAC AATGTCGGTACAATGCCAAC G
siRNAs
FOLR1	GGA UGU UUC CUA CCU AUA UdTdT	AUA UAG GUA GGA AAC AUC CdTdT
MDM2	GAA AAU UCA GAU GAA UUA UdTdT	AUA AUU CAU CUG AAU UUU CdTdT
PHB1	GCA AAG AUU UAC AGA AUG UdTdT	ACA UUC UGU AAA UCU UUG CdTdA
PHB2	CAG AAU CGU AUC UAU CUC AdTdT	UGA GAU AGA UAC GAU UCU GdTdT
Scrambled	GUA CUC AUG CUA UAU UGC UdTdT	AGC AAU AUA GCA UGA GUA CdTdT