FOLR1
|
TTCATCCAGGACACCTGCCTC |
ATTGCTCACAGTCCTCTTTGC |
MDM2
|
AACAGGTGTCACCTTGAAGGTG |
TGAGGTAGATGGTCTAGAAACC |
GAPDH
|
TGGTAAAGTGGATATTGTTGC |
TTCTCAGCCTTGACGGTGC |
Genotyping PCR primers
|
FOLR1
|
TCAGGTGATCCACCCACCTC |
AGATCTTTGGAGGAGTCATTC |
Guide RNAs
|
Sense (5′‐3′)
|
Antisense (5′‐3′)
|
FOLR1‐1 |
ACACC ACCTGAACCTCGTGACCACC G |
AAAAC GTGGTCACGAGGTTCAGGTC G |
FOLR1‐2 |
ACACC GTTGGCATTGTACCGACATT G |
AAAAC AATGTCGGTACAATGCCAAC G |
siRNAs
|
FOLR1
|
GGA UGU UUC CUA CCU AUA UdTdT |
AUA UAG GUA GGA AAC AUC CdTdT |
MDM2
|
GAA AAU UCA GAU GAA UUA UdTdT |
AUA AUU CAU CUG AAU UUU CdTdT |
PHB1
|
GCA AAG AUU UAC AGA AUG UdTdT |
ACA UUC UGU AAA UCU UUG CdTdA |
PHB2
|
CAG AAU CGU AUC UAU CUC AdTdT |
UGA GAU AGA UAC GAU UCU GdTdT |
Scrambled |
GUA CUC AUG CUA UAU UGC UdTdT |
AGC AAU AUA GCA UGA GUA CdTdT |