TABLE 2.
Antibodies and dilution conditions
| Antibodies | Company | Application | Dilution |
|---|---|---|---|
| PHB1 (#2426) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| PHB2 (E1Z5A, #14085) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| p53 (#9282) | Cell Signaling Technology | IB | 1/5000 in TBS containing 0.2% Tween‐20 and 10% Block Ace |
| MDM2 (D1V2Z, #86934) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| Rb (D20, 9313) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| Phospho–histone H3 (Ser10) (#3377, D2C8) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| Phospho‐Wee1 (Ser642) (#4910, D47G5) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| CDK2 (#2546, 78B2) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| CDK4 (#12790, D9G3E) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| CDK6 (#3136, DCS83) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| Myt1 (#4282) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| p27(Kip1) (#3686, D69C12) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| Cyclin D3 (#2936, DCS22) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| p21(Waf1/Cip1) (#2947, 12D1) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| Cyclin A2 (#4656, BF683) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| Cyclin B1 (#12231, D5C10) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| Phospho‐CDC2 (Tyr15) (#4539, 10A11) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| Cyclin E2 (#4132) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| p18(Ink4c) (#2896, DCS118) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| Cyclin D1(#2978, 92G2) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| Phospho‐GSK3β (Ser9) (#9323, 5B3) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| EP300 (#86377, D8Z4E) | Cell Signaling Technology | IB | 1/1000 in Immunoenhancer |
| FOLRα (NCL‐L‐FRα ) | Leica | IB | 1/1000 in Immunoenhancer |
| Phospho‐ATM (Ser1981) (2152‐1) | Epitomics | IB | 1/1000 in Immunoenhancer |
| GAPDH (016‐25523, 5A12) | FujiIFilm Wako | IB | 1/1000 in Immunoenhancer |
| HRP‐conjugated goat anti–mouse IgG (G0407) | Tokyo Chemical Industry | IB | 1/1000 in Immunoenhancer |
| HRP‐conjugated goat anti–rabbit IgG (G0418) | Tokyo Chemical Industry | IB | 1/1000 in Immunoenhancer |
| Phospho p38 (Thr180/Tyr182, #4511) | Cell Signaling Technology | IB | 1/3000 in Immuno‐enhancer |
| p38 (#9212) | Cell Signaling Technology | IB | 1/5000 in Immuno‐enhancer |
| Phospho Erk1/2 | Cell Signaling Technology | IB | 1/3000 in Immuno‐enhancer |
| Erk1/2 (#4695) | Cell Signaling Technology | IB | 1/5000 in Immuno‐enhancer |
| Phospho Akt (Ser473, #4060) | Cell Signaling Technology | IB | 1/3000 in Immuno‐enhancer |
| Akt (#9272) | Cell Signaling Technology | IB | 1/5000 in Immuno‐enhancer |
| Caspase‐3 (#9662) | Cell Signaling Technology | IB | 1/3000 in Immuno‐enhancer |
| FOLRα (NCL‐L‐FRα) | Leica | IP for IB | 3 μg |
| MDM2 (sc‐813, N‐20) | Santa Cruz Biotechnology | IP for IB | 3 μg |
| Normal human IgG (143‐09501) | FujiFilm Wako | IP for IB | 3 μg |
| Normal rabbit IgG (PM035) | MBL | IP for IB | 3 μg |
| FOLRα (NCL‐L‐FRα) | Leica | IP for MS | 1 μg |
| Normal human IgG (143‐09501) | FujiFilm Wako | IP for MS | 1 μg |
| FOLRα (NCL‐L‐FRα) | Leica | Flow cytometry /FACS | 10 μg/mL |
Abbreviations. IB, immunoblot analysis; IP, immunoprecipitation; MS, mass spectrometry; FACS, fluorescence‐activated cell sorting.