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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Nat Protoc. 2021 Jan 11;16(2):754–774. doi: 10.1038/s41596-020-00432-x

Fig. 2 ∣. Overview of VAMPIRE analysis, from the extraction of contour coordinates to the automatic generation of shape modes.

Fig. 2 ∣

a, The contour of a single cell described by 50 equidistant points along its contour. b, Unaligned (left) shapes of a set of cells are pooled, normalized by size, and aligned (right). c, Eigenshape vectors (i.e., principal components or PCs) are obtained from a principal component analysis (PCA) of the contour coordinates of aligned cells. d, Reconstructed cell shape from a reduced number of eigenshape vectors. The reduced number of eigenshape vectors was defaulted to the number of vectors that comprise 95% of the shape variations among all assessed cells. e, Representative cellular shape modes are obtained by applying a K-means clustering method to a set of cell morphology data described by the reduced number of eigenshape vectors.