Skip to main content
. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Nat Protoc. 2021 Jan 11;16(2):754–774. doi: 10.1038/s41596-020-00432-x

Fig. 3 ∣. Overview of VAMPIRE implementation with the VAMPIRE GUI.

Fig. 3 ∣

a, The VAMPIRE Graphical User Interface (GUI). b, Flow diagram illustrating key steps in the implementation of VAMPIRE analysis with VAMPIRE GUI. Images of cells are first segmented into binary images that highlight the cellular region and/or nuclear region. The VAMPIRE GUI top section (highlighted in red) allows users to specify analysis parameters and the location of segmented images to be used to create a VAMPIRE analysis model. Once the VAMPIRE analysis model is established, the user can specify the sets of segmented images to be analyzed using the previously established model (highlighted in blue).