Skip to main content
. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Nat Protoc. 2021 Jan 11;16(2):754–774. doi: 10.1038/s41596-020-00432-x

Fig. 8 ∣. Analysis of nuclear shape in H&E stained tissue sections with VAMPIRE.

Fig. 8 ∣

a, Images of a skin tissue section stained with hematoxylin and eosin (H&E) and obtained from the cancer genome atlas (TCGA case ID: TCGA-EE-A20I). Nuclei in the epidermis and the reticular dermis regions were segmented and analyzed with VAMPIRE. b, Bar graphs show the distribution of nuclei shape modes, comparing epidermal cells (N = 1,579) and dermal cells (N = 498) using VAMPIRE analysis. Numbers above the bars represent the abundances (%) of nuclei in each shape mode. Results also show a lower Shannon entropy in cells derived from the reticular dermis (S = 2.1) relative to cells from the epidermis (S = 2.25), indicating lower heterogeneity in the reticular dermis.