Figure 3.

Q277G right-shifts SSD without altering activation.(A) Outside-out patch recording of cASIC1 Q277G responses to increasingly acidic solutions. (B) Responses of Q277G to pH 5.5 application when preincubated with solutions ranging from pH 8.0 to 6. Note solutions of intermediate acidity (pH 6.8–6.4) produce persistent currents at equilibrium. (C) Response curves to activation (solid triangles) or SSD (open circles) for WT (black) or Q277G (red). (D) Mean ± SEM pH₅₀s of activation (left) and SSD (right) for WT (black) or Q277G (red). Fits from individual patches are shown as symbols following the legend for C. All recordings done at −60 mV holding potential. Error bars depict SEM.