Figure 6.

Human ASIC1a Q276G also does not abolish desensitization.(A) Whole-cell recordings of hASIC1a WT or Q276G, on either the G212 (left) or D212 (right) background during pH 5.5 (red trace) or 6.5 (light red trace) application from baseline pH 8.0. The inset shows the D212 variant traces on expanded time base. (B) Summary of desensitization time constants (left) and percent of steady-state current (right) at pH 5.5 (circles) and 6.5 (triangles) for WT (black) and Q277G (red) in the indicated background. The decay of Q276G in D212 background required double exponential fits. The fast component (78–79% of area) and slow components are reported. The percent of steady state is relative to the peak current of the test pH. Symbols denote single cells, and error bars are SEM. Holding potential is −60 mV.