Video 2.
Representative trajectory illustrating N277 from a single chain (cyan carbon atoms) and the surrounding residues (from top right and clockwise: E412, L414, E80, and E417; gray carbon atoms) in the Q277N mutant cASIC1. N277 is highly dynamic and rarely forms hydrogen bonds to E80Oε and L414O. E80 is deprotonated, while E412 and E417 are protonated. The animation shows 100 ns of simulation time. Non-polar hydrogen atoms are omitted. Playback speed, 3 ns/s.