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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Sci Transl Med. 2021 Feb 3;13(579):eabb6576. doi: 10.1126/scitranslmed.abb6576

Fig. 4. IL-33 signaling through ST2 in eosinophils protects against hepatic IR injury.

Fig. 4.

(A) Serum concentrations of IL-33 were measured in C57BL/6 and ST2−/− mice at various times after reperfusion (n = 4 in sham group, n = 6 in all remaining groups). (B to F) WT and IL-33−/− mice were subjected to hepatic IR surgery. Hepatic eosinophil accumulation, shown as proportions among CD45+ nonparenchymal cells, was measured at 4 hours after reperfusion (n = 8 in WT and n = 6 in IL-33−/− group) (B). Serum concentrations of ALT (C) and AST (D) were measured at 4 and 8 hours after reperfusion. Liver necrosis (scale bars, 200 μm) was examined at 24 hours after reperfusion (E) and quantified (F) (n = 4 in WT and n = 3 in IL-33−/− group). (G to J) IL-33−/− mice were adoptively transferred with WT-bmEos, IL-33–stimulated WT-bmEos, or PBS as control at 24 hours before hepatic IR surgery (n = 3 in IL-33−/− + PBS, n = 4 in all remaining groups). Serum concentrations of ALT (G) and AST (H) were measured at 4 and 8 hours after reperfusion. Liver necrosis (scale bars, 200 μm) was examined at 24 hours after reperfusion (I) and quantified (J). A two-tailed unpaired Student’s t test with Welch’s correction was performed in (F). A one-way ANOVA was performed in (J). A two-way ANOVA was performed in (A), (C), (D), (G), and (H).