Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 28;220(8):e202012104. doi: 10.1083/jcb.202012104

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Yang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure S3. — The ESCRT machinery is essential for the degradation of plasma protein Hxt3 during glucose starvation.(A) Western blots showing the degradation of Hxt3-GFP after glucose starvation in the indicated strains. Asterisk indicates a minor cleavage product from full-length protein. (B) Quantification (±SD, n = 3) of protein levels in A. (C–H) Subcellular localization of Hxt3-GFP before (0 h) and after (3 h) glucose starvation in WT (C), vps4Δ (D), vps27Δ (E), vps23Δ (F), vps36Δ (G), and snf7Δ (H) cells. (I and J) Colocalization of Hxt3-GFP with Hse1-mCherry (I) and Vps4-3HA-mCherry (J) during glucose starvation. Dashed lines indicate the periphery of yeast cells. Scale bars, 3 µm.