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. 2021 May 28;220(8):e202012104. doi: 10.1083/jcb.202012104

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© 2021 Yang et al.

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Figure S5. — Purified vacuoles do not contain a functional ubiquitination system.(A) Comparison of protein levels between whole-cell lysate (Wcl) and purified vacuoles (Vac). Samples that contain a similar level of Vph1 were loaded in each lane. (B) Quantification (±SD, n = 3) of the relative protein levels in A, which was normalized to Vph1.