Video 2.
Rapamycin treatment triggers the degradation of Zrt3*-GFP, Cot1-GFP, Fth1-GFP, but not Zrc1-GFP, related to Fig. 1. All four proteins were expressed from a TET-OFF plasmid. Mid-log cells expressing one of the four proteins were loaded into a microfluidic imaging chamber and allowed to recover for 2 h (-2 h). 30 min (-30 min, 2 h for Fth1) before the addition of rapamycin, doxycycline was perfused into the chamber to stop the transcription. The time of adding rapamycin was recorded as 0 h The rapamycin treatment took 6–9 h. Pictures were taken every 30 min. During the treatment, several punctate structures (highlighted by white arrows) were observed. The video plays at 2 frames per second.