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Fth1 is not constitutively degraded, related to Fig. 2. Mid-log cells expressing Fth1-GFP was loaded into a microfluidic imaging chamber and allowed to grow in a fresh YNB medium. After a brief recovery (30 min), images were taken every 2 min for 4 h with 2% laser power. No increase of lumenal free GFP was observed, despite the fact that vacuoles undergo normal fusion (highlighted by white arrows) and fission processes. Scale bar, 2 µm. The video plays at 8 frames per second.
