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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Cancer Lett. 2021 Apr 1;509:1–12. doi: 10.1016/j.canlet.2021.03.026

Figure 2. Role of FASN in mediating PPI effect.

Figure 2.

A, Western blot analysis of FASN and PARP1 expression in MCF7 cells with ectopic FASN over-expression and in drug resistant MCF7/AdVp3000 (M3K) cells with FASN knockdown. B, Relative resistance factor (RRF) to lansoprazole in MCF7 cells with FASN over-expression or M3K cells with FASN knockdown. RRF=Lansoprazole IC50(FASN or Sh)/IC50(Vec or Scr). C, Western blot analysis of endogenous FASN and PARP1 expression in MCF7, T47D, MDA-MB-231, and MDA-MB-468 cells following treatments with lansoprazole at different concentrations. D, Effect of lansoprazole on NHEJ activity in MCF7 and MDA-MB-468 cells. E, Rescue of lansoprazole inhibition of NHEJ activity by ectopic FASN over-expression. F, Western blot analysis of γH2AX level in MCF7 and MDA-MB-468 cells treated with lansoprazole at different concentrations. G, Ablation of lansoprazole-induced γH2AX increase by ectopic FASN over-expression as determined using Western blot analysis. Actin was used as a loading control for all Western blot analyses.