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TIMS mobility distribution of CTB pentamer with GM1s ligand bound with mobility selection for CID and SID experiments highlighted with gray slice (A). Reduced mobilities were calculated using the protein/protein complex calibration. TIMS-CID and TIMS-SID of mobility-selected 14+ (B, C) with corresponding deconvolution to show variations of GM1s retention on subunits (B2, C2). CID and SID pathways of CTB with GM1s and the observed products (D, E).
