. Author manuscript; available in PMC: 2022 Apr 22.

Published in final edited form as: J Med Chem. 2021 Apr 12;64(8):4333–4358. doi: 10.1021/acs.jmedchem.0c01686

Table 3.

Cellular Assays and Conditions

Assay	Protein	Readout	Reference
Luc reporter	GAL4-BCL6^BTB	Luciferase catalysis	60, 91
M2H	^aGAL4-BCL6^BTB, VP16^b	Luciferase catalysis	38, 42, 50, 78
NanoBRET	full length BCL6 and corepressor	HL Fluorescence	95
FACS	eGFP-BCL6^BTBc	eGFP / mCherry	83

GAL4-BCL6^BTB = GAL4-BCL6^BTB(5-129)

VP16 = VP16-BCOR(112-753)

eGFP, enhanced green fluorescent protein.