FIGURE 3.

Linear time course of body water deuterium enrichment (%) following oral bolus of 100 mL D₂O (70 atoms %) in participants preintervention (A) and postintervention (B) in the subcohort of participants who underwent the MyoPS measurements. Values are means ± SDs; n = 28. The bolus was consumed on day −3 before the intervention began and on day 165 of supplementation. (C) The integrated myofibrillar FSR (%/d) pre- and postintervention. Each line represents 1 participant (n = 7 in CON, n = 9 in LEU-PRO, n = 5 in LEU-PRO+n–3). Data from participants for whom paired measurements were not available (e.g., MyoPS data only available either pre- or postintervention) are not shown. Data were analyzed by an intention-to-treat approach using a linear mixed model with group (CON, LEU-PRO, LEU-PRO+n–3) as a fixed factor, participant as a random factor and preintervention values as covariates. Data from participants with both paired (pre and post) and unpaired (pre or post) MyoPS data were included in the analysis as per the intention-to-treat approach (at preintervention: n = 9 in CON, n = 12 in LEU-PRO and n = 6 in LEU-PRO+n–3; at postintervention: n = 7 in CON, n = 9 in LEU-PRO and n = 6 in LEU-PRO+n–3). There were no differences in MyoPS between groups (P > 0.05). CON, control; D₂O, deuterated water; FSR, fractional synthetic rate; LEU-PRO, leucine-enriched protein; LEU-PRO+n–3, leucine-enriched protein plus long-chain n–3 PUFAs; MyoPS, myofibrillar protein synthesis; PRE, preintervention; POST, postintervention.