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. Author manuscript; available in PMC: 2021 Aug 12.
Published in final edited form as: J Am Chem Soc. 2020 Jul 29;142(32):13779–13794. doi: 10.1021/jacs.0c04226

Figure 2.

Figure 2.

Difference spectra of the full anaerobic reconstitution of CuACcP at pH 7.4 by addition of 2.5 mol equiv of Cu(II) at room temperature. The initial spectrum of the apoprotein, which contains ~5% hemoprotein, has been subtracted. Arrows indicate the trajectory of absorbance changes over the course of 400 s, and asterisks denote isosbestic points. Spectra of two distinct species are shown in the inset. The green T2Cu intermediate species and stable purple species are shown in close approximations of their respective apparent colors with absorption peaks indicated.