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. 2021 May 13;54(6):e13055. doi: 10.1111/cpr.13055

FIGURE 5.

FIGURE 5

Enhancing Tie2 phosphorylation can effectively alleviate activated MCs‐induced ECs proliferation. A, p‐Tie2 and Tie2 expressions on ECs were detected by Western blot. B, Qualifications of EdU‐positive rates in D. C, Qualifications of EdU‐positive rates in E. D, E, EdU was used to detect ECs proliferation. Results are presented as the mean values (±SD), *P < .05, **P < .01. Scale bars, 50 μm. Tie2, TEK tyrosine kinase. MCs, mesangial cells. ECs, endothelial cells. Angpt2, angiopoietin2. Angpt1, angiopoietin. PDGF‐BB, platelet‐derived growth factor BB, to activate MCs. (A, B, D) Control, ECs co‐cultured with inactive MCs. PDGF‐BB, ECs co‐cultured with PDGF‐BB‐activated MCs. Angpt2, ECs co‐cultured with inactive MCs and Angpt2, was added. PDGF‐BB+Angpt1, ECs co‐cultured with PDGF‐BB‐activated MCs and Angpt1, was added. (C, E) Control, normal ECs. NC, ECs transfected with negative control siRNA. si‐Tie2, ECs transfected with si‐Tie2