FIGURE 6.

Enhancing Tie2 phosphorylation can effectively alleviate ECs proliferation in anti‐Thy‐1 nephritis. A, PAS staining showed cell number per glomerulus. RECA‐1 and PCNA expressions in glomeruli were detected by immunohistochemical staining. B, Locations of RECA‐1 and PCNA expressions were showed by immunofluorescence co‐staining. White arrows indicated proliferating ECs. RECA‐1, red. PCNA, green. DAPI, blue. C, Hypercellularity index per glomerulus was evaluated (n = 7). D, RECA‐1 area staining rate per glomerulus was qualified (n = 7). E, PCNA‐positive cells rate per glomerulus was qualified (n = 7). Results are presented as the mean values (±SD), *P < .05, **P < .01. Scale bars, 50 μm. ECs, endothelial cells; PCNA, proliferative cells nuclear antigen; RECA‐1, rat endothelial cells antigen‐1; Tie2, TEK tyrosine kinase. Control, normal rats. Model, anti‐Thy‐1 nephritis rats. Vasculotide, anti‐Thy‐1 nephritis rats treated by Vasculotide