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Temin and Rubin's focus assay for morphologic transformation by Rous sarcoma virus. (Left) Experimental scheme. (Center) A transformed focus visualized by phase contrast microscopy. (Right) Relationship between viral concentration and number of foci. (Center and right panels from Temin H, Rubin H. 1958. Virology 6: 669–688, with permission from Elsevier.)
