Stable and monodisperse PLGA NDs co-encapsulate prostratin and aCD25. (a) ND hydrodynamic diameters were measured by DLS; gray: Blank NDs, blue: aCD25-NDs, yellow: Pro-NDs, green: Pro-aCD25-NDs. (b) SEM images of Pro-aCD25-NDs at increasing magnifications (top to bottom). Scale bar = 2 μm (top) and 1 μm (bottom), (c) Absorbance spectra were analyzed using UV–Vis–NIR spectroscopy, (d) Zeta potential was measured by electrophoretic light scattering. The encapsulation efficiency of (e) prostratin was determined by absorbance spectroscopy and that of (f) aCD25 was determined by BCA protein assay.