Figure 1.

Transcriptional analysis of CD4⁺ T cells from peripheral blood and decidua. (A) Heatmap result of an unsupervised hierarchical clustering of genes that is significantly different (p < 0.01) in dCD4⁺ T cell samples from RSA compared with dCD4⁺ T cells from NP (left). Heatmap result of differential gene in samples of dCD4⁺ T cells compared with samples of pCD4⁺ T cells from NP (right). Each column represents a patient (blue: NP-dCD4⁺ T cells, red: RSA- dCD4⁺ T or NP-pCD4⁺ T cells), and each row represents a gene. The heatmap indicates the level of row normalized gene expression. Red = high expression; Blue = low expression. (B) GO analysis of differentially expressed genes. (C) 51 upregulated and 54 downregulated transcriptional factor genes in 2826 differentially expressed genes of early RSA dCD4⁺ T cells. And 279 upregulated and 96 downregulated transcriptional factor genes in 1621 differentially expressed genes of NP dCD4⁺ T cells. (D) Volcano plot and (E) Venn diagram of differentially expressed transcriptional factor genes; orange and purple points mark the genes with significantly increased or decreased expression, respectively, in samples of dCD4⁺ T cells from RSA compared with dCD4⁺ T cells from NP (FDR < 0.01, left). Volcano plot in samples of dCD4⁺ T cells compared with samples of pCD4⁺ T cells from NP (FDR < 0.01, right). The x-axis shows Log₂ (Fold Change) in expression, and the y-axis shows the Log₁₀ (Adjusted p-value) of a gene being expressed differentially. In both data sets, NR2F2 is the top-ranked gene.