Table 3C.

Lymphoid tissue Treg enriched more exosome pathways than non-lymphoid tissue Treg. A. Exosome gene numbers in benign disease Treg groups.

Datasets	Category	Treg source	Type	TotalDEG	Exo up	/DEG%	Exo down	/DEG%
GSE116347	Lymphoid	Tumor bearing mouse (B16) spleen	RNA-seq	1218	328	26.93	113	9.28
GSE116347	Lymphoid	Tumor bearing mouse (MC38) spleen	RNA-seq	1298	331	25.50	147	11.33
GSE116347	Lymphoid	Tumor bearing mouse (CT26) spleen	RNA-seq	905	236	26.08	92	10.17
GSE120280	Lymphoid	Tumor bearing mouse (TC-1) spleen	RNA-seq	1776	475	26.75	158	8.90
GSE139372	Non-lymphoid	Melanoma Skin	RNA-seq	1571	121	7.70	287	18.27
GSE120280	Non-lymphoid	Tumor bearing mouse (TC-1) tumor	RNA-seq	1933	419	21.68	270	13.97
GSE89225	Non-lymphoid	Breast Tumor	RNA-seq	1493	204	13.66	250	16.74
GSE116347	Non-lymphoid	Tumor bearing mouse (B16) tumor	RNA-seq	2462	572	23.23	375	15.23
GSE116347	Non-lymphoid	Tumor bearing mouse (MC38) tumor	RNA-seq	1651	415	25.14	268	16.23
GSE116347	Non-lymphoid	Tumor bearing mouse (CT26) tumor	RNA-seq	1393	188	13.50	376	26.99

IPA analysis was performed to identify upregulated commonly shared exosome pathways in malignant disease tissue Treg (cut off: p < 0.05 and |Z-score| >= 2). A total of 69 exosome pathways enriched in malignant Treg groups, including 35 commonly shared pathways and 34 specific pathways. The 42 of 69 exosome pathways were identified in the lymphoid tissue Treg. Vitamin-C Transport and Endothelin-1 Signaling were the most common enriched pathways. Cell Cycle Control of Chromosomal Replication, Cell Cycle Control of Chromosomal Replication, BEX2 Signaling Pathway, Cholesterol Biosynthesis III (via Desmosterol), Superpathway of Cholesterol Biosynthesis, Cholesterol Biosynthesis II (via 24,25-dihydrolanosterol), Cholesterol Biosynthesis I and Glycolysis I were only commonly shared in non-lymphoid tissue Treg.

Exo, exosome; DEG, differentially expressed gene; DEG%, percentage in total DEG numbers.