Table 3D.

Gene numbers of caspase-4 dependent GSDMD secretomes were more than caspase-1 dependent GSDMD secretomes in various Treg groups.

Datasets	Category	Treg source	Type	Total DEG	Casp-1up	/DEG%	Casp-1down	/DEG%	Casp-4 up	/DEG%	Casp-4 down	/DEG%
GSE119169	Lymphoid	Spleen	Array	44	0	0.00	0	0.00	2	4.55	0	0.00
GSE37532	Lymphoid	Lymph node	Array	397	8	2.02	0	0.00	12	3.02	0	0.00
E-MTAB-7961	Lymphoid	Spleen	RNA-seq	1802	15	0.83	3	0.17	35	1.94	9	0.50
GSE20366	Non-lymphoid	Small intestinal lamina propria	Array	756	16	2.12	11	1.46	15	1.98	11	1.46
GSE64909	Non-lymphoid	Brown adipose tissue	Array	113	8	7.08	0	0.00	8	7.08	0	0.00
GSE37532	Non-lymphoid	Visceral adipose tissue(VAT)	Array	924	18	1.95	2	0.22	22	2.38	5	0.54
E-MTAB-7961	Non-lymphoid	Kidney	RNA-seq	1584	20	1.26	4	0.25	42	2.65	5	0.32
GSE50096	Lymphoid	Skeletal muscle injured 2w spleen	Array	308	6	1.95	0	0.00	10	3.25	1	0.32
GSE50096	Lymphoid	Skeletal muscle injured 4d spleen	Array	313	7	2.24	0	0.00	13	4.15	1	0.32
E-MTAB-7961	Non-lymphoid	Fibrosis kidney	RNA-seq	2440	13	0.83	4	0.25	37	2.36	8	0.51
E-MTAB-7961	Non-lymphoid	Regeneration kidney	RNA-seq	2365	25	1.06	6	0.25	56	2.37	7	0.30
GSE50096	Non-lymphoid	Skeletal muscle injured 2w muscle	Array	539	10	1.86	0	0.00	11	2.04	5	0.93
GSE50096	Non-lymphoid	Skeletal muscle injured 4d muscle	Array	465	10	2.15	2	0.43	10	2.15	8	1.72
GSE116347	Lymphoid	Tumor bearing mouse (B16) spleen	RNA-seq	1218	20	1.64	3	0.25	35	2.87	3	0.25
GSE116347	Lymphoid	Tumor bearing mouse (MC38) spleen	RNA-seq	1298	18	1.39	2	0.15	42	3.24	4	0.31
GSE116347	Lymphoid	Tumor bearing mouse (CT26) spleen	RNA-seq	905	14	1.55	1	0.11	28	3.09	2	0.22
GSE120280	Lymphoid	Tumor bearing mouse (TC-1) spleen	RNA-seq	1776	21	1.18	9	0.51	38	2.14	11	0.62
GSE139372	Non-lymphoid	Melanoma Skin	RNA-seq	1571	2	0.13	8	0.51	8	0.51	19	1.21
GSE120280	Non-lymphoid	Tumor bearing mouse (TC-1) tumor	RNA-seq	1933	18	0.93	6	0.31	33	1.71	13	0.67
GSE89225	Non-lymphoid	Breast Tumor	RNA-seq	1493	9	0.60	6	0.40	16	1.07	18	1.21
GSE116347	Non-lymphoid	Tumor bearing mouse (B16) tumor	RNA-seq	2462	42	1.71	9	0.37	72	2.92	23	0.93
GSE116347	Non-lymphoid	Tumor bearing mouse (MC38) tumor	RNA-seq	1651	27	1.64	11	0.67	39	2.36	21	1.27
GSE116347	Non-lymphoid	Tumor bearing mouse (CT26) tumor	RNA-seq	1393	9	0.65	16	1.15	11	0.79	52	3.73

GSDMD, Gasdermin-D; Casp-1, caspase-1; Casp-4, caspase-4; DEG, differentially expressed gene.

However, the total numbers in each Treg datasets was too low to perform IPA analysis. The detailed lists were shown in Supplementary Table 7 .