Table 3E.

Metascape analysis was performed to identify pathways associated with upregulated caspase-1 dependent GSDMD secretome gene lists in various Treg groups.

Enrichment	normal spleen	normal tissue	disease spleen	disease tissue	tumor spleen	tumor tissue
Pathways in cancer	+	+	+	+	+	+
vesicle organization	+		+		+	+
negative regulation of apoptotic signaling pathway		+			+	+
myeloid leukocyte activation	+				+
striated muscle cell differentiation		+		+
cell morphogenesis involved in differentiation		+		+
supramolecular fiber organization		+				+
extracellular structure organization			+			+
response to reactive oxygen species				+		+
cell-substrate junction assembly				+		+
Fluid shear stress and atherosclerosis					+	+
positive regulation of organelle organization	+
ECM-receptor interaction	+
NABA MATRISOME ASSOCIATED		+
morphogenesis of an epithelium		+
extracellular matrix disassembly		+
TNF signaling pathway		+
establishment of organelle localization		+
wound healing		+
Smooth Muscle Contraction		+
regulation of lymphocyte differentiation		+
response to peptide		+
angiogenesis		+
regulation of cell adhesion		+
Hypertrophic cardiomyopathy (HCM)		+
Diseases of signal transduction by growth factor receptors and second messengers		+
actomyosin structure organization				+
Extracellular matrix organization				+
organophosphate biosynthetic process				+
branching morphogenesis of an epithelial tube				+
epithelial cell development				+
Hemostasis				+
cellular response to external stimulus				+
carbohydrate metabolic process				+
Non-integrin membrane-ECM interactions				+
response to oxidative stress					+
post-translational protein modification					+
coenzyme metabolic process					+
cell-substrate adhesion					+
cellular response to oxygen levels					+
generation of precursor metabolites and energy					+
positive regulation of cell motility					+
MAPK signaling pathway					+
Degradation of the extracellular matrix					+
Proteoglycans in cancer					+
regulation of body fluid levels					+
regulation of cellular protein localization						+
positive regulation of binding						+
PID INTEGRIN4 PATHWAY						+
Post-translational protein phosphorylation						+
cell division						+
positive regulation of response to external stimulus						+
positive regulation of cell migration						+
chromatin remodeling						+
regulation of cholesterol metabolic process						+
response to hypoxia						+
purine nucleoside triphosphate metabolic process						+
Cell Cycle, Mitotic						+