Figure 2.

RUNX1-EVI1 expression causes reduced cell cycling and colony forming capacity in hematopoietic progenitors. (A) Fewer floating hematopoietic progenitor (HC) cells were present in day 2 blast culture, following induction of RUNX1-EVI1, n=5, **P<0.01. Cell cycle stages, n=3. (B) and quiescence, n=5 (C) were assessed in the whole blast culture at the same time-point, showing an increased proportion of cells in G₀ and G₁. Example flow cytometry plots are shown to the right, *P< 0.05, **P<0.01. (D) Floating progenitor cells with RUNX1-EVI1 induced formed fewer colonies, n=3, **P<0.01 (E) Colonies following doxycycline (dox) induction in blast culture only were comprised of approximately equivalent proportions of granulocyte/macrophage (GM), erythroid (Ery) or mixed colonies, with a slight increase in the mixed-type at the expense of singular lineage, n=3. All error bars (A to E) represent standard error of the mean. (F) Representative brightfield images of colonies with and without dox induction in blast culture only.