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. 2020 Apr 30;106(6):1659–1670. doi: 10.3324/haematol.2019.233924

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Copyright© 2021 Ferrata Storti Foundation

This article is distributed under the terms of the Creative Commons Attribution Noncommercial License (by-nc 4.0) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

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Figure 4. — The impact of E2 and E4 on hematopoietic stem and progenitor cells in an in vitro model of the human hematopoietic niche. (A) Total hematopoietic cells after 1 week of co-culture with human bone marrow mesenchymal stromal cells (BM-MSC) in the presence of estrogens. (B) Total hCD34+ cells after 1 week of co-culture with human BM-MSC in the presence of estrogens. (C) Colony-forming units (CFU) derived from hematopoietic stem and progenitor cells after 1 week of co-culture with human BM-MSC in the presence of estrogens. Data were obtained from three to six biological replicates and are presented as the mean ± standard deviation. The statistical significance was analyzed by one-way analysis of variance with the Fisher least significant difference test: *P<0.05 and **P<0.01.