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. 2020 May 28;106(6):1624–1635. doi: 10.3324/haematol.2019.239202

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Figure 5. — F8 shortened promoters drive green fluorescent protein expression in hepatic endothelial cells. Immunofluorescence analyses of C57BL/6 livers 2 weeks after lentiviral vector (LV) LV.pF8-600.GFP, LV.pF8-446.GFP or LV.pF8.342.GFP delivery, compared to the full length LV.pF8-1175.GFP. (A) Representative pictures of liver sections stained for the endothelial marker (Lyve-1, in red) and the green fluorescent protein (GFP) using 400x magnification. (B) Liver of LV-injected mice decorated with the macrophage marker F4/80 (red) in combination with GFP (green). Scale bars, 50 mM. Nuclei are stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). n=3-5 (mice); sections=3-6 per mouse. (for antibodies specifications refers to the Online Supplementary Appendix).