. Author manuscript; available in PMC: 2021 Sep 1.

Published in final edited form as: Nat Protoc. 2021 Jan 29;16(3):1761–1784. doi: 10.1038/s41596-021-00491-8

Table 1.

Restriction enzymes for validation of seven SARS-CoV-2 plasmids.

Plasmid	Restriction enzyme(s)	DNA fragments with expected size
pUC57-CoV-2-F1	BsaI	3644 bp (F1)^a + 1367 bp + 1366 bp
pCC1-CoV-2-F2	BsaI	6466 bp + 3886 bp (F2)^a + 1344 bp
pCC1-CoV-2-F3	BsaI	6466 bp + 4480 bp (F3)^a + 1344 bp
pUC57-CoV-2-F4	BsaI	5607 bp (F4)^a + 1367 bp + 1366 bp
pUC57-CoV-2-F5	Esp3I and PvuI	4457 bp (F5)^a + 1674 bp + 620 bp + 234 bp + 161 bp+ 42 bp
pUC57-CoV-2-F6	Esp3I and PvuI	4284 bp (F6)^a + 1674 bp + 620 bp + 234 bp + 161 bp+ 42 bp
pCC1-CoV-2-F7	Esp3I and SnaBI	3563 bp (F7)^a + 2522 bp + 2395 bp + 1689 bp + 651 bp + 553 bp

Fragment of SARS-CoV-2 genome