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| Fragment | gF1 | gF2 | gF3 | gF4 | gF5 | gF6 | gF7 | gF8 | gF9 |
|---|---|---|---|---|---|---|---|---|---|
| Forward primer (in 10 μM) | cov-1V | cov-3225V | cov-7382V | cov-11707V | cov-14618V | cov-18037V | cov-21521V | cov-25068V | cov-27875V |
| Reverse primer (in 10 μM) | pncov-R1 | pncov-F2m-R | pncov-R3 | cov-14995R | cov-18377R | pncov-R5 | cov-25238R | cov-28099R | pncov-R7 |
| Annealing temperature | 58°C | 58°C | 59°C | 59°C | 54°C | 55°C | 55°C | 55°C | 58°C |
| Amplicon size in base-pair | 3.6 kb | 4.3 kb | 4.6 kb | 3.2 kb | 3.8 kb | 4.02 kb | 3.7kb | 3.0 kb | 2.0 kb |
