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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Nat Protoc. 2021 Jan 29;16(3):1761–1784. doi: 10.1038/s41596-021-00491-8
Sequence of 9 pairs of primers for PCR
Primer name Sequence (5’–3’)
cov-1V ATTAAAGGTTTATACCTTCCCAGG
pncov-R1 GGGCCGACAACATGAAGACAGTG
cov-3225V CTGTTGGTCAACAAGACGG
pncov-F2m-R CTATTACGTTTGTAACACATCATACATGTAGATGAATTAC
cov-7382V CAAATGGCCCCGATTTCAG
pncov-R3 CAAAGGCTTCAGTAGTATCTTTAGC
cov-11707V AGTTTCTACACAGGAGTTTAG
cov-14995R TGGAAAACCAGCTGATTTGTC
cov-14618V CTACGTGCTTTTCAGTAG
cov-18377R GTAGAAAAACCTAGCTGTAAAGG
cov-18037V AAGCTGAAAATGTAACAGG
pncov-R5 TCGCACTAGAATAAACTCTGAACTC
cov-21521V TGTTATTTCTAGTGATGTTCTTG
cov-25238R CAATCAAGCCAGCTATAAAACC
cov-25068V TCTCTGGCATTAATGCTTC
cov-28099R GATTTAGAACCAGCCTCATCC
cov-27875V TTGTCACGCCTAAACGAAC
pncov-R7 TTTTTTTTTTTTTGTCATTCTCCTAAGAAGC