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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Nat Protoc. 2021 Jan 29;16(3):1761–1784. doi: 10.1038/s41596-021-00491-8
Reaction systems for plasmid validation.
Components Volume (μl)
Nuclease-free water Up to 10
10×Cutsmart buffer 1
Restriction enzymes 0.5–1a
DNA Plasmid TBD (200–300 ng)
a
Overall volume of the enzyme should be <10% of the total reaction volume.