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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Nat Protoc. 2021 Jan 29;16(3):1761–1784. doi: 10.1038/s41596-021-00491-8
Restriction enzyme digestion reactions for preparing large amounts of DNA fragments.
Components Volume (μl)
Nuclease-free water Up to 50 μl
10×Cutsmart buffer 5 μl
Restriction enzymes 1 unit/μga
Plasmids TBD (30 μg)
a
The total volume should be <10% of the reaction volume.