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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Nat Protoc. 2021 Jan 29;16(3):1761–1784. doi: 10.1038/s41596-021-00491-8
Calculation of volume of each DNA fragment required for ligation
Fragment Size (kb) Mass of each fragment for assembling 1 μg full-length DNA Mass of each fragment for assembling 5 μg of full-length DNA (μg)
F1 3644 0.12179 0.61
F2 3886 0.12988 0.65
F3 4480 0.14973 0.75
F4 5607 0.18739 0.935
F5 4457 0.14896 0.745
F6 4284 0.14318 0.715
F7 3565 0.11915 0.595
Total 29921 1 5