PCR reactions for amplifying N-gene
| Components | Volume (μl) |
|---|---|
| Nuclease-free water | Top up to 50 |
| 2× Platinum™ SuperFI™ II PCR master mix | 25 |
| Forward primer CoV-T7-N-F | 2.5 |
| Reverse primer polyT-N-R | 2.5 |
| Template DNA (Plasmid pCC1-CoV-2-F7) | TBD (1–10 ng) |
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