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| Components | For transcribing full-length RNA | For transcribing N RNA |
|---|---|---|
| 2×NTP/CAP | 25 μl | 10 μl |
| GTP | 7.5 μl | 0.75 μl |
| 10× reaction buffer | 5 μl | 2 μl |
| DNA template | Up to 7.5 μl (use 1–2 μg) | TBD (use 1 μg) |
| Enzyme Mix | 5 μl | 2 μl |
| Nuclease-free water | Top up to 50 μl | Top up to 20 μl |
