Figure 2.

ML264 inhibits H/R-induced fibrosis of H9C2 cells. A-B, invasion and migration abilities of H9C2 cells determined by Transwell assays (one-way ANOVA** p < 0.01 vs. Control ^## p < 0.01 vs. H/R treatment); C-D, mRNA (c) and protein (d) expression of fibronectin, Vimentin, α-SMA and Collagen I/II in cells determined by RT-qPCR and western blot analysis, respectively (two-way ANOVA, * p < 0.05, ** p < 0.01 vs. Control, ^# p < 0.05 vs. H/R treatment); E, Vimentin and α-SMA expression in cells further evaluated by immunofluorescence staining (one-way ANOVA, ** p < 0.01 vs. Control, ^## p < 0.01 vs. H/R treatment); F, total collagen content in cells determined by a collagen kit (one-way ANOVA, ** p < 0.01 vs. Control, ^# p < 0.05 vs. H/R treatment). Data were exhibited as mean ± SD from at least three independent experiments