Figure 3.

miR-27a is significantly up-regulated in H9C2 cells following ML264 treatment. A, a volcano map for differentially expressed miRNAs in cells following ML264 treatment; B, top 15 differentially expressed miRNAs; C, miR-27a expression in cells determined by RT-qPCR (one-way ANOVA, ** p < 0.01 vs. Control, ^## p < 0.01 vs. H/R treatment); D, conservative sequence of KLF5; E, binding sites between KLF5 and the promoter region of miR-27a predicted on JASPAR; F, binding relationship between KLF5 and the promoter region of miR-27a validated through a ChIP-qPCR analysis (two-way ANOVA, ** p < 0.01 vs. anti-IgG). Data were exhibited as mean ± SD from at least three independent experiments