Figure 4.

miR-27a mimic suppresses H/R-induced apoptosis and fibrosis in H9C2 cells. miR-27a mimic was introduced in H/R-treated cells while miR-27a inhibitor was transfected into ML264-treated cells. A, miR-27a expression in cells determined by RT-qPCR (one-way ANOVA, ** p < 0.01 vs. Mock, ^## p < 0.01 vs. ML264 + miR-27a inhibitor); B-C, viability of cells determined by EdU labeling (b) and CFSE labeling (c) assays (one-way ANOVA, ** p < 0.01 vs. Mock, ^## p < 0.01 vs. ML264 + miR-27a inhibitor); D-E, apoptosis of H9C2 cells determined by Hoechst 33342/PI double staining (d) and flow cytometry (e) (one-way ANOVA, ** p < 0.01 vs. Mock, ^## p < 0.01 vs. ML264 + miR-27a inhibitor); F-G, mRNA (f) and protein (g) expression of Vimentin and α-SMA in cells determined by RT-qPCR and western blot analysis, respectively. Data were exhibited as mean ± SD from at least three independent experiments (two-way ANOVA, * p < 0.05 vs. Mock, ^# p < 0.05 vs. ML264 + miR-27a inhibitor). Data were exhibited as mean ± SD from three independent experiments